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The antibody is from purified rabbit serum by affinity purification by using sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene has actually been observed in lots of tumors, which results in chromosome instability, and thus may possibly contribute to tumorigenesis. This protein was identified to affiliate with, and become involved in, the phosphorylation of NPAT protein (nuclear protein mapped to your ATM locus), which participates in mobile-cycle regulated histone gene expression and plays a significant position in endorsing cell-cycle progression within the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinct isoforms, happen to be described. Two more splice variants ended up claimed but detailed nucleotide sequence information and facts is not really nonetheless out there.
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Blocking peptides are peptides that bind specifically to your focus on antibody and block antibody binding. These peptide generally has the epitope identified from the antibody.
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This information outlines even more achievable optimization of mobile and immunoassay click here parameters to be sure the best possible effects are...
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This guidebook outlines even further possible optimization of mobile and immunoassay parameters to be certain the absolute best final results are...
Antibodies certain to the blocking peptide no more bind to your epitope to the focus on protein. By comparing the staining from the blocked antibody compared to the antibody by yourself, you can see which staining is particular.
Focus on Information and facts Cyclin E belongs to your highly conserved cyclin relatives, whose customers are characterised by a remarkable periodicity in protein abundance with the mobile cycle. Cyclins operate as regulators of CDK kinases. Unique cyclins exhibit distinctive expression and degradation designs which lead into the temporal coordination of every mitotic function. Cyclin E types a complex with and functions like a regulatory subunit of CDK2, whose exercise is necessary for cell cycle G1/S changeover. This protein accumulates within the G1-S stage boundary and is particularly degraded as cells development by way of S stage.
The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by both antibodies...
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